This page contains the publicly available protocols developed to date with EDEN funding.

Some protocols were developed by EDEN exchange participants (graduate students, postdocs, or faculty). Other protocols were developed by undergraduate students who held EDEN summer internships (indicated with an asterisk *).

We hope to eventually make these protocols accessible via a searchable database. For now, they are simply grouped roughly into taxonomic groups based on the origanisms the protocols were designed for. Please browse through them to find ones that might be applicable to your work!

If you have protocols optimized for use in non-traditional organisms that you would be willing to make available here, please email them in PDF format to edenrcn@fas.harvard.edu with the subject line "Protocol."

Plant Protocols

Genetic transformation of bunchberry dogwood (Cornus canadensis) using Agrobacterium Suspension. Developed by Chris Trlica (North Carolina State University) during a summer internship in the Xiang lab.*

Spore germination and sex ration determination in moss. Developed by Tatum Norrell (University of Florida) during a summer internship in the McDaniel lab.*

Genetic transformation of moss (Ceratodon purpureus). Developed by Pierre-Francois Perroud of the Quatrano lab during an exchange visit to the McDaniel lab.

Vertebrate Protocols

Stickleback (Gasterosteus) trypsin cleared alizarin and alican double stain. Developed by Brian Lohman (University of Idaho) during a summer internship in the Bell lab.*

Stickleback (Gasterosteus) whole-mount antibody staining with trypsin clearing. Developed by Brian Lohman (University of Idaho) during a summer internship in the Bell lab.*

Deer mouse (Peromyscus) spectrometry measurement and analysis of fur Brightness. Developed by Pavitra Muralidhar (Cornell University) during a summer internship in the Hoekstra lab.*

Invertebrate Protocols

Alcian blue staining for horseshoe crab (Limulus) Paraffin-Embedded Sections. Developed by Leslie Slota (University of Florida) during a summer internship in the Cohn lab.*

Immunohistochemistry for hyaluronic acid in horseshoe crabs (Limulus). Developed by Leslie Slota (University of Florida) during a summer internship in the Cohn lab.*

In situ hybridization on horseshoe crab (Limulus) Tissue Sections. Developed by Leslie Slota (University of Florida) during a summer internship in the Cohn lab.*

Horseshoe crab (Limulus) whole mount in situ hybridization. Developed by Leslie Slota (University of Florida) during a summer internship in the Cohn lab.*

Immunohistochemistry for tardigrade embryos (Hypsibius). Developed by Frank Smith of the Jockusch lab during an exchange visit to the Goldstein lab.

In situ hybridization for tardigrade embryos (Hypsibius). Developed by Frank Smith of the Jockusch lab during an exchange visit to the Goldstein lab.

Blastomere ablations in amphipod (Parhyale) embryos. Developed by Frederike Alwes of the Extavour lab during an exchange visit to the Averof lab.

Making transgenic amphipods (Parhyale). Developed by Frederike Alwes of the Extavour lab during an exchange visit to the Averof lab.

Heat shock treatment for amphipods (Parhyale). Developed by Frederike Alwes of the Extavour lab during an exchange visit to the Averof lab.

In situ hybridization on snail embryos (Ilyanassa). Developed by Bishoy Kamel of the Medina lab during an exchange visit to the Lambert lab.

Making tools for manipulating slipper limpet embryos (Crepidula). Developed by Maryna Lesoway of the Collin lab during an exchange visit to the Henry lab.

Collection and fixation of slipper limpet embryos (Crepidula). Developed by Maryna Lesoway of the Collin lab during an exchange visit to the Henry lab.

In situ hybridization on slipper limpet embryos (Crepidula). Developed by Maryna Lesoway of the Collin lab during an exchange visit to the Henry lab.

Immunostaining on slipper limpet embryos (Crepidula). Developed by Maryna Lesoway of the Collin lab during an exchange visit to the Henry lab.

Other Protocols

Choanoflaggelate RNA extraction. Developed by Parinaz Fozouni (University of California, Berkeley) during a summer internship in the King lab.*

Cloning choanoflaggelate PCR products. Developed by Parinaz Fozouni (University of California, Berkeley) during a summer internship in the King lab.*

Tools for handling high-throughput sequence data. I. Cloud Computing with Amazon Web Services. Developed by Dacotah Melicher of the Bowsher lab during an exchange visit to the Dworkin lab.

Tools for handling high-throughput sequence data. II. Creating Storage Volumes For Large Files. Developed by Dacotah Melicher of the Bowsher lab during an exchange visit to the Dworkin lab.

Tools for handling high-throughput sequence data. III. Descriptions of Common Scripts and Workflow. Developed by Dacotah Melicher of the Bowsher lab during an exchange visit to the Dworkin lab.

Tools for handling high-throughput sequence data. VI. Quick and Effective Side-by-Side Installation of Linux on a Windows PC. Developed by Dacotah Melicher of the Bowsher lab during an exchange visit to the Dworkin lab.